Journal: PeerJ

Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting

doi: 10.7717/peerj.20583

Figure Lengend Snippet: (A) Green fluorescent protein detection in LN229 and U87MG cell lines. (B) The detection of expression level of ASIC2 by shRNA plasmid. (C) Detection of ASIC2 expression level by shRNA plasmid. (D) Detection of ASIC2 expression level by overexpression plasmid. (E) Detection of cell proliferation ability with U87MG cells. (F) Detection of cell proliferation ability of LN229 cells in each group. (G) Detection of cell clone forming ability of U87MG and LN229 cells. (H) Statistical analysis of colony formation in U87MG and LN229 cells. (I) Detection of cell invasion ability of U87MG and LN229 cells. (J) Statistical analysis of the number of U87MG and LN229 cells migrating through the chamber. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01 vs control, # P < 0.05, ## P < 0.01 vs plasmid control.

Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the U87MG cell line was acquired from the American Type Culture Collection (ATCC).

Techniques: Expressing, shRNA, Plasmid Preparation, Over Expression, Control

Journal: PeerJ

Article Title: The role of ASIC2 in glioma progression: implications for prognosis and therapeutic targeting

doi: 10.7717/peerj.20583

Figure Lengend Snippet: (A) Detection of cell migration ability of glioma groups. (B) Statistical analysis of the wound closure rate in U87MG cells. (C) Statistical analysis of the wound closure rate in LN229 cells. (D–E) Regulation of cell cycle by ASIC2 on LN229 cells. (F, H) ASIC2 regulates the expression levels of P21, cycline D1 proteins in LN229. (G, I) Statistical analysis of protein expression in LN229 cells after ASIC2 interference and overexpression. (J–K) ASIC2 regulates the expression levels of MMP2, calcineurin and NFAT1 proteins in glioma cells. (L–O) Statistical analysis of protein expression in U87MG and LN229 cells after ASIC2 interference and overexpression. Data are expressed as the mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001 vs control, # P < 0.05, ## P < 0.01, ### P < 0.001 vs plasmid control.

Article Snippet: The human glioma cell line LN229 was obtained from the Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, and the U87MG cell line was acquired from the American Type Culture Collection (ATCC).

Techniques: Migration, Expressing, Over Expression, Control, Plasmid Preparation

Journal: Cell Communication and Signaling : CCS

Article Title: EF24 targets METTL3 to reprogram m6A methylation and induce ferroptosis: an epitranscriptomic mechanism with therapeutical potential for glioma

doi: 10.1186/s12964-025-02583-4

Figure Lengend Snippet: EF24 inhibits glioma cell proliferation. A CCK-8 assay showing the inhibitory effect of EF24 on glioma cell viability and the IC50 values for LN229 and A172 cells. B Plate colony formation assay assessing the effect of EF24 on glioma cell proliferation. C Immunofluorescence analysis of the effect of EF24 on glioma cell proliferation (magnification ×200, scale bar: 50 μm). Note: * P < 0.05, ** P < 0.01, *** P < 0.001 compared to the control group

Article Snippet: Human glioma cell lines LN229 (RRID: CVCL-0393) and A172 (RRID: CVCL-0131) were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA), cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin antibiotic cocktail (10,000 U/mL penicillin, 10 mg/mL streptomycin).

Techniques: CCK-8 Assay, Colony Assay, Immunofluorescence, Control

Journal: Cell Communication and Signaling : CCS

Article Title: EF24 targets METTL3 to reprogram m6A methylation and induce ferroptosis: an epitranscriptomic mechanism with therapeutical potential for glioma

doi: 10.1186/s12964-025-02583-4

Figure Lengend Snippet: METTL3 inhibits the ferroptosis in glioma cells. A Western blotting analysis of NRF2 and GPX4 in LN229 and A172 cells in the negative control group and the METTL3 knockdown group. B Correlation analysis of METTL3 expression with NRF2 and GPX4 mRNA levels in GBM tissue specimens. C Detection of intracellular malondialdehyde (MDA) levels after METTL3 knockdown. D Intracellular glutathione (GSH) levels following METTL3 knockdown. E TEM images showing mitochondrial morphological changes in METTL3-knockdown cells (magnification: ×7000, scale bar = 2.0 μm; magnification: ×20000, scale bar = 50 μm). F Detection of glioma proliferation following METTL3 knockdown by colony formation assay. Note: * P < 0.05, ** P < 0.01, *** P < 0.001 versus the control group

Article Snippet: Human glioma cell lines LN229 (RRID: CVCL-0393) and A172 (RRID: CVCL-0131) were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA), cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin antibiotic cocktail (10,000 U/mL penicillin, 10 mg/mL streptomycin).

Techniques: Western Blot, Negative Control, Knockdown, Expressing, Colony Assay, Control

Journal: Cell Communication and Signaling : CCS

Article Title: EF24 targets METTL3 to reprogram m6A methylation and induce ferroptosis: an epitranscriptomic mechanism with therapeutical potential for glioma

doi: 10.1186/s12964-025-02583-4

Figure Lengend Snippet: METTL3 recruits YTHDF1 to regulate NRF2 mRNA stability. A Actinomycin assay showing the effect of YTHDF1 on NRF2 stability. B qRT-PCR showing the effect of knockdown of YTHDF1 on NRF2 expression. C Correlation analysis of YTHDF1 and NRF2 expression in GBM tissue specimens. D Western blotting showing the effect of knockdown of YTHDF1 on NRF2 expression. E RIP-qPCR revealing the binding of YTHDF1 with NRF2 in stable METTL3 knockdown and negative control LN229 and A172 cells. Note: * P < 0.05, ** P < 0.01, *** P < 0.001 compared to control

Article Snippet: Human glioma cell lines LN229 (RRID: CVCL-0393) and A172 (RRID: CVCL-0131) were procured from the American Type Culture Collection (ATCC; Manassas, VA, USA), cultured in Dulbecco’s Modified Eagle Medium (DMEM, Gibco, Thermo Fisher Scientific) supplemented with 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin-streptomycin antibiotic cocktail (10,000 U/mL penicillin, 10 mg/mL streptomycin).

Techniques: Quantitative RT-PCR, Knockdown, Expressing, Western Blot, Binding Assay, Negative Control, Control